Regulation of trophoblast invasion by IL‐1β and TGF‐β1
S Karmakar, C Das - American Journal of Reproductive …, 2002 - Wiley Online Library
S Karmakar, C Das
American Journal of Reproductive Immunology, 2002•Wiley Online LibraryKarmakar S, Das C. Regulation of trophoblast invasion by IL‐1β and TGF‐β1. AJRI 2002;
48: 210–219© Blackwell Munksgaard, 2002 PROBLEM: To investigate the effect of IL‐1β
and TGF‐β1 on trophoblast derived proteases and their inhibitors at the mRNA and protein
level for elucidation of the mechanism of trophoblast invasion. METHOD OF STUDY:
Trophoblast derived proteases and their inhibitors were localized in human placental villi by
immunohistochemistry, their regulation at mRNA and protein levels were studied using RT …
48: 210–219© Blackwell Munksgaard, 2002 PROBLEM: To investigate the effect of IL‐1β
and TGF‐β1 on trophoblast derived proteases and their inhibitors at the mRNA and protein
level for elucidation of the mechanism of trophoblast invasion. METHOD OF STUDY:
Trophoblast derived proteases and their inhibitors were localized in human placental villi by
immunohistochemistry, their regulation at mRNA and protein levels were studied using RT …
Karmakar S, Das C. Regulation of trophoblast invasion by IL‐1β and TGF‐β1. AJRI 2002; 48:210–219 © Blackwell Munksgaard, 2002
PROBLEM: To investigate the effect of IL‐1β and TGF‐β1 on trophoblast derived proteases and their inhibitors at the mRNA and protein level for elucidation of the mechanism of trophoblast invasion.
METHOD OF STUDY: Trophoblast derived proteases and their inhibitors were localized in human placental villi by immunohistochemistry, their regulation at mRNA and protein levels were studied using RT–PCR and zymography, respectively, on trophoblast cells in culture.
RESULTS: Trophoblast proteases, matrix metalloproteases (MMP‐2, MMP‐9), membrane type matrix metalloproteases (MT‐MMP1&2) and urokinase type plasminogen activator (uPA) were found to be up regulated by IL‐1β while the protease inhibitors, tissue inhibitor of metalloproteases (TIMP‐1 & 2) and plasminogen activator inhibitors (PAI‐1 & 2) to be up regulated by TGF‐β1.
CONCLUSION: Temporo‐spatial regulation of trophoblast invasion is the outcome of a balanced interplay between the proteases and their cognate inhibitors. IL‐1β and TGF‐β1 seem to be critical for regulating the protease network thereby effectively controlling the extent to which the trophoblast may invade the maternal endometrium.
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